WebDL-草丁膦;草丁膦;Basta®;DL-草铵膦; ... 制果株式会社(Japan Meiji Seika Kaisha)开发。双丙氨膦中有活性的植物毒性成分是草丁膦(Phosphinothricin, PPT)。后来由德国Hoechst AG Frankfurt公司商业化销售化学合成的草丁膦(Phosphinothricin, PPT),通用名称为:草铵膦 ... WebSep 1, 2016 · Increasing the transformation efficiency is essential and is being carried out using biolistics, Agrobacterium and microinjection of protoplasts methods and selection …
Antagonistic Interaction between Phosphinothricin and
WebSep 12, 2015 · Phosphinothricin Sensitivity of Screening Transgenic plants were screened for the expression of the bar gene by using 10 % ( v / v) commercial herbicide Basta (Sangon Biotech Co., Ltd). For the selection of optimal concentration for the transformants’ growth, various concentrations of Basta were tested. WebMar 3, 2015 · The T-DNA from these vectors confers phosphinothricin (Basta) resistance to plants. Gateway-compatible destination vectors for tissue-specific expression After creating pMCS:GW and pMCS:YFP-GW, we cloned the presumptive promoters of several genes with previously described expression patterns (Table 1 ) into these vectors to create a set of ... income efiling india
Characterization of the herbicide-resistance gene bar from
WebThe level of resistance to the commercial preparation of phosphinothricin (Basta; Roussel-Hoechst Agrovet) was evaluated by in vitro and in vivo assays. Using in vitro selective conditions for phosphinothricin, only plantlets from four kanamycin-resistant independent lines remained green and continued to grow and root. WebDescription DL-Phosphinothricin is a herbicide that has been used as a selection agent for the genes bar or pat and is the active ingredient in Basta®, Glufosinate Ammonium, PPT, … WebJun 9, 2024 · Basta herbicide resistance assay For Basta herbicide resistance assays, seeds were sown as above and 10 to 14 day old wildtype or Basta resistant seedlings grown in 2.5 inch pots were treated with 25 ml of 3 mg/l Basta, results were collected 7 days after exposure. Agrobacterium mediated leaf-infiltration for transient gene expression incentive\\u0027s wf