Phospho-rps6

WebCells were then intracellularly stained with 2 µg/mL of purified anti-RPS6 Phospho (Ser244) antibody (clone A18024A) overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective. WebRibosomal protein S6 (rpS6 or eS6) is a component of the 40S ribosomal subunit and is therefore involved in translation. Mouse model studies have shown that phosphorylation …

National Center for Biotechnology Information

WebFeb 6, 2024 · We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully... WebProduct name Anti-RPS6 (phospho S235) antibody [EP1338 (2)Y] - Ribosome Marker See all RPS6 primary antibodies Description Rabbit monoclonal [EP1338 (2)Y] to RPS6 (phospho S235) - Ribosome Marker Host species Rabbit Tested applications Suitable for: WB, IP, IHC-P, Dot blot more details Unsuitable for: Flow Cyt or ICC/IF Species reactivity ionz software https://loudandflashy.com

Recombinant Anti-RPS6 (phospho S235 + S236) antibody [SP50] …

Web上海士锋生物为您提供rere抗体的参数及2024年最新报价，厂家专业的rere抗体售后服务团队，是您值得信赖的合作伙伴 WebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) Immunofluorescence staining of phospho-S6 (red) and TFE3 (green) in control (NTC) cells and cells lacking TSC1 or TSC2. Cells were starved or starved and restimulated with ... WebQuality Control: the Phospho-RPS6 (S240/S244) assay kit is routinely tested against Insulin-treated MCF7 lysates. MCF7 cells were cultured in a T175 flask to 90% confluence and stimulated with 10 µM of insulin for 60 min at RT. Following cell lysis using 16 mL of 1X Lysis Buffer 4, lysates ionz gamer edition

DRAM1 regulates autophagy and cell proliferation via inhibition of …

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Phospho-rps6

Purified anti-RPS6 Phospho (Ser244) Antibody - BioLegend

WebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster … WebMar 9, 2024 · Anti-RPS6 (phospho S235 + S236) antibody [SP50] See all RPS6 primary antibodies Description Rabbit monoclonal [SP50] to RPS6 (phospho S235 + S236) Host species Rabbit Tested applications Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt more details Species reactivity Reacts with: Mouse, Human Immunogen

Phospho-rps6

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WebNational Center for Biotechnology Information WebMar 9, 2024 · Levels of phosphorylated RPS6 (p-RPS6) and S6 kinases (p-S6K) are shown to indicate the effectiveness of rapamycin at inhibiting mTORC1 activity.

WebMay 3, 2024 · (i) Ribosomal protein S6 (rpS6) is a downstream substrate of PI3K/akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double strand breaks. Phospho-rpS6 is reliably measurable by flow cytometry and thus has the potential to function as a biomarker of responsiveness to several therapeutic agents. WebRPS6 is a 249 amino acid protein with a predicted molecular weight of 28.6 kD. Distribution Cytosol, nucleus, ER, ubiquitous expression. Function Phosphorylation events at a cluster …

WebFeb 6, 2024 · RPS6 is phosphorylated downstream of active EGF signaling 13 and is lost within the first 24 hours of AG1478 treatment (Supplementary Fig. 4A). Quantification of … WebThe phosphorylation of ribosomal protein S6 (rpS6) has been described for the first time about four decades ago. Since then, numerous studies have shown that this modification …

WebMar 9, 2024 · Flow Cytometry - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691) Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial …

WebNational Center for Biotechnology Information on the line movie 2022 freeWebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also … ion 代码Webp-rpS6 is a robust post-treatment indicator of HER2 pathway-targeted therapy resistance; Resistance to Selumetinib (AZD6244) in colorectal cancer cell lines is mediated by … on the line mel gibson endingWebRPS6 Rabbit Polyclonal Antibody: 50μl: 593.00元: 加入购物车: AG3129: Phospho-S6 Ribosomal Protein (Ser235/236) Mouse Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3133: Phospho-S6 Ribosomal Protein (Ser235/Ser236) Rabbit Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3136: Phospho-S6 Ribosomal Protein (Ser240/244 ... on the line movie 2022 castWebPhospho-S6 Ribosomal Protein (Ser240, Ser244) Antibody (MA5-37141) in Flow Flow cytometry of Phospho-S6 Ribosomal Protein (Ser240, Ser244) using K562 cells. Samples … ion zupcu photographerWebWB analysis using 80206-1-RR. Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80206-1-RR (Phospho-S6 Ribosomal protein (Ser236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. on the line movie 2022 rotten tomatoesWebResults: The activity of a downstream effector of the HER2 pathway phosphorylated ribosomal protein S6 (p-rpS6), was suppressed by trastuzumab in the parental cell lines … on the line movie 2022 wiki